Prevention of chromosomal breakage in Fanconi's anemia by cocultivation with normal cells
✍ Scribed by I. Nordenson; B. Björksten; B. Lundh
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 213 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0340-6717
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Cocultivation of fibroblast cells from a male patient with Bloom syndrome (BS) and a female control reduced the rate of sister chromatid exchanges in the BS cells from a mean of 54 SCE per metaphase (range 42--65) to 41 (range 24--59). Medium used to culture control cells for 48 h also reduced the r
The incidence of structural chromosome aberrations and the rate of sister chromatid exchanges (SCE) was investigated in lymphocyte cultures from a patient with typical Fanconi's anemia and his parents. The rate of SCEs was found to be normal. In experiments with the alkylating agent Trenimon the SCE