Abstract
Staphylococcus epidermidis, a main etiologic agent of implant‐related infections, is showing increasing resistance to several antibiotic substances, among them the aminoglycosides, a class of drugs playing a relevant role in current medical protocols to prevent and treat clinical infections. Here we investigated the prevalence of aac(6′)‐Ie‐aph(2″), aph(3′)‐IIIa, and ant(4′) genes, encoding for the three forms of aminoglycoside‐modifying enzymes (AME), responsible for resistance to aminoglycoside antibiotics, in 70 clinical isolates of S. epidermidis from orthopedic postsurgical and implant‐related infections. In addition, ermA and ermC, the two most common staphylococcal genes conferring antibiotic resistance to macrolides, lincosamides, and streptogramin B (MLS~B~) were included in this investigation. All isolates were characterized by automated ribotyping, so that the presence of antibiotic resistance determinants was investigated in strains exhibiting different ribopatterns. Interestingly, combinations of coexisting AME genes appeared to be typical of specific ribopatterns. The aac(6′)‐Ie‐aph(2″) gene was the most prevalent AME gene, being observed in 44% of the isolates. As far as the determinants for MLS~B~ antibiotics are concerned, the ermC gene was observed in 33% of the isolates, while ermA was detected in a single isolate. These results provide a detailed characterization in terms of antibiotic resistance determinants of clones of S. epidermidis frequently isolated from implant orthopedic infections, providing useful indications for more effectual future strategies of infection prevention/eradication based on the incorporation of antibiotic drugs in biomaterials. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009