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Pretreatment of wheat straw using combined wet oxidation and alkaline hydrolysis resulting in convertible cellulose and hemicellulose

✍ Scribed by Anne Belinda Bjerre; Anne Bjerring Olesen; Tomas Fernqvist; Annette Plöger; Anette Skammelsen Schmidt


Book ID
102650779
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
923 KB
Volume
49
Category
Article
ISSN
0006-3592

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✦ Synopsis


The wet oxidation process of wheat straw has been studied as a pretreatment method to attain our main goal: To break down cellulose t o glucose enzymatic, and secondly, to dissolve hemicellulose (e.g., for fermentation) without producing microbial inhibitors. Wet oxidation combined with base addition readily oxidizes lignin from wheat st raw faci I it at i ng the pol y sacc ha rides for enzymatic hydrolysis. By using a specially constructed autoclave system, the wet oxidation process was optimized with respect to both reaction time and temperature. The best conditions (20 g/L straw, 170°C, 5 to 10 min) gave about 85% w/w yield of converting cellulose t o glucose. The process water, containing dissolved hemicellulose and carboxylic acids, has proven t o be a direct nutrient source for the fungus Aspergillus niger producing exo-p-xylosidase. Furfural and hydroxymethyl-furfural, known inhibitors of microbial growth when other pretreatment sys- tems have been applied, were not observed following the wet oxidation treatment.