Pretargeting using peptide nucleic acid

✍ Scribed by M. Rusckowski; Timothy Qu; F. Chang; Donald J. Hnatowich

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 161 KB
• Volume: 80
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19971215)80:12+<2699::aid-cncr48>3.0.co;2-4

✦ Synopsis

Tc. Target localization of the PNA-bound protein in a mouse infection and a Massachusetts.

mouse tumor model occurred by passive diffusion while the radiolabeled complementary PNA localized by in vivo hybridization. The PNA-streptavidin was prepared by adding biotin-conjugated PNA to streptavidin; the complementary PNA, derivatized with a primary amine, was conjugated with acetyl S-protected NHS-MAG 3 bifunctional chelator and radiolabeled with 99m Tc.

RESULTS.

In both the infection and tumor mouse models, increased localization of radiolabel was achieved in animals receiving both injectates compared with control animals receiving only the radiolabeled PNA. In the infection model, the infected to normal thigh radioactivity ratio was 3.5 for the study animals compared with 1.7 for control animals (P Å 0.0001). In the tumor model, these values were 1.7 versus 1.2 (P Å 0.003).

CONCLUSIONS.

We conclude that PNA may be considered an alternative to (strept)avidin and biotin for pretargeting studies.