Pressurized flow driven electrochromatography using fluorinated-bonded silica

✍ Scribed by C. Chaiyasut; T. Tsuda; S. Kitagawa; H. Wada; T. Monde; Y. Nakabeya

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 109 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:8<590::aid-mcs4>3.0.co;2-g

✦ Synopsis

Capillary electrochromatography using fluorinated-bonded silica Fluo-ᮋ . fix under pressurized flow was demonstrated to be applied to the separation of charged compounds and neutral compounds. The reduction and extension of the elution time due to the application of high voltage were demonstrated. The typical variation of elution order of the samples was observed. The reduction of electroos-Ž . motic flow mobility from the linearity was comparatively observed on the osm two different effluents when the voltage was applied to the column. The alternations of retention factors of neutral compounds due to applied voltage are observed and the retention mechanisms of the compounds are discussed. With the same electrochromatographic condition and pH of the effluents used, the elec-Ž . troosmotic flow velocity V magnitude of the phosphate buffer effluent was osm higher than the ammonium acetate effluent system. Thus the ammonium acetate might suppress the potentially enhanced negative charge of the stationary phase.