✦ LIBER ✦

Pressurized CEC with gradient elution for separation of flavonoids from corn

✍ Scribed by Shujuan Wang; Li Jia; Deping Chen

Book ID: 102441734
Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 238 KB
Volume: 32
Category: Article
ISSN: 1615-9306
DOI: 10.1002/jssc.200800494

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A pressurized CEC (pCEC) method with gradient elution was developed for the analysis of flavonoids in two genetically modified corns and five nongenetically modified corns. The pCEC separation of flavonoids was performed on a monolithic silica‐ODS column with UV detection at 270 nm. The effects of the concentration of organic solvent in the mobile phase, pH of the electrolyte buffer, applied voltage, and the gradient elution conditions were evaluated systematically. Gradient elution mode was used as an online concentration method to improve the detection sensitivity of flavonoids. Compared to the conventional injection (0.2 μL), there were 9‐ to 20‐fold improvements in the detection sensitivity at the injection volume of 5 μL without adverse effect on the band broadening and the separation resolution. The LODs for the six flavonoids were in the range of 6.5–46 ng/mL under the gradient elution conditions. The developed gradient pCEC method was applied to evaluate flavonoids in various types of corns. The contents of flavonoids in genetically modified corns and nongenetically modified corns were compared.

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