𝔖 Bobbio Scriptorium
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Pressure–temperature stability of DNA in neutral salt solutions

✍ Scribed by S. A. Hawley; R. M. Macleod

Book ID
102762556
Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
617 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The effect of pressure on the melting temperature of DNA (Cl. perfringens) has been examined in several concentrations of neutral salt by measurement of uv absorbance. The results indicate that the apparent transition volume increases as the salt concentration, and hence the melting temperature, is raised, and suggest that the transition occurs without a change in volume at a T~m~ of 59°C. Additional experiments were conducted in an effort to determine whether transition behaviour can be found in other regions of pressure and temperature; no additional transition behaviour was observed in experiments conducted with temperatures as low as −21.85°C at 2000 atm and pressures in excess of 9000 atm at 58°C.

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