Pressure affects enzyme function in organic media
β Scribed by Jungbae Kim; Jonathan S. Dordick
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 477 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0006-3592
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The catalytic activities of lyophilized powders of β£-chymotrypsin and Candida antarctica lipase were found to increase 4-to 8-fold with increasing amounts of either buffer salts or potassium chloride in the enzyme preparation. Increasing amounts of sorbitol in the chymotrypsin preparation produced a
Catalytic activities of β£-chymotrypsin and subtilisin Carlsberg in various hydrous organic solvents were measured as a function of how the enzyme suspension had been prepared. In one method, lyophilized enzyme was directly suspended in the solvent containing 1% water. In another, the enzyme was prec