Preservation of Trehalose-Loaded Red Blood Cells by Lyophilization
✍ Scribed by Török, Zsolt; Satpathy, Gyana R.; Banerjee, Mitali; Bali, Rachna; Little, Erika; Novaes, Roberta; Ly, Hung Van; Dwyre, Denis M.; Kheirolomoom, Azadeh; Tablin, Fern; Crowe, John H.; Tsvetkova, Nelly M.
- Book ID
- 120619615
- Publisher
- Mary Ann Liebert
- Year
- 2005
- Tongue
- English
- Weight
- 332 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1538-344X
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Two different methods commonly used to preserve intact yeast cells-freezing and freeze-dryingwere compared. Different yeast cells submitted to these treatments were stored for 28 days and cell viability assessed during this period. Intact yeast cells showed to be less tolerant to freeze-drying than
PP-50, a synthetic pH-responsive biopolymer, is here shown to increase the permeability of the phospholipid bilayer to trehalose, a disaccharide accumulated in desiccation tolerant organisms across all kingdoms. Uptake of 251 AE 6 mM intracellular trehalose facilitated an increase in the membrane in