Presentation of Leishmania donovani promastigotes occurs via a brefeldin A-sensitive pathway
✍ Scribed by Thierry Lang; Paul M. Kaye
- Book ID
- 102826947
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 828 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
For the presentation of Leishmania promastigotes to polyclonal CD4+ Tcells, a processing period within activated macrophages of 3-4 h is required. Presentation can be inhibited by both chloroquine and brefeldin A (BFA), the latter implicating a requirement for newly synthesized MHC class I1 molecules. This inhibition is both reversible and specific, in that BFA did not inhibit mixed lymphocyte reaction stimulation by these infected macrophages. Immunogold labeling demonstrated that class I1 was associated with the parasite-containing phagolysosome. The level of class I1 was not significantly altered in BFA-treated cells in the time period studied, suggesting that antigen may exist the phagolysosome and interact with class I1 in another cellular compartment.