Presentation of alloantigens by host cells

✍ Scribed by Rosemary A. Sherwood; Leslie Brent; Lee S. Rayfield

• Book ID: 102826416
• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 570 KB
• Volume: 16
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830160519

✦ Synopsis

Presentation of alloantigens by host cells

Presentation of alloantigens by host cells has been examined in vivo by means of a murine cell transfer system. Primary (1") hosts were activated by the i.p. administration of allogeneic spleen cells and their spleen or peritoneal cells were transferred into syngeneic secondary (2") hosts 3 days later. Sensitization of 2" hosts was assessed by their ability to reject donor strain skin grafts prematurely. The transferred cells were routinely depleted of T lymphocytes. We show that (a) 5 x lo7 spleen and 3 x lo6 peritoneal cells consistently caused marked accelerated graft rejection; (b) this effect was antigen specific and observable in all strain combinations studied; (c) it was caused by the active sensitization of 2" hosts, but not by contaminating donor strain cells; (d) the cells involved were plastic adherent and viability was not a requirement; and (e) both class I and 11, but not minor, histocompatibility antigens played a role in this model. We conclude that presentation of alloantigens by host antigen-presenting cells can be a potent route of allosensitization.