Presence of gelatinase A and etalloelastase type protease at the plasma membrane of human skin fibroblasts. Influence of cytokines and growth factors on cell-associated metalloendopeptidase levels
✍ Scribed by Jean-Yves Beranger; Gaston Godeau; Camille Frances; Ladislas Robert; William Hornebeck
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 496 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
ABSTRACT
Gelatinase A and elastase type proteinase (Homsy, et al., 1988) present at plasma membranes of human skin fibroblasts (HSF) were separated by anion exchange chromatography on a DEAE Tris acryl M column. Elastase type proteinase (HSFE1) was able to convert 72 kDa progelatinase A to a lower 66 kDa M.W. active enzyme. Several cytokines (IL‐1β, 1L4, IL6), interferon γ (IFN γ) and tumor growth factor β (TGFβ) were studied for their ability to modify the levels of those plasma membrane associated proteinases. Among these mediators, only IL‐1β was found to enhance the amounts of HSF membrane‐bound HSFE~1~ and Gelatinase A.