Presence of CD8α-CD8β-positive TcR γ/δ thymocytes in the fetal murine thymus and their in vitro expansion with interleukin-7
✍ Scribed by Georges Leclercq; Magda De Smedt; Jean Plum
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 517 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Several groups have described that a low percentage of in vitro cultured Tcell receptor (TcR) yl6 cells express CD8. Contrary toTcR alp cells, however, CD8 on these TcR y/6 cells was shown to be a CD8a homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vitro culture of fetal day 14 thymic lobes in an organ culture system or of fetal day 18 fetal thymocytes in cell suspension yields CD8P-positive TcR y/6 cells. This is not the result of IL-7-induced expression of CD8P on previously CD8p-negative cells. It is due to IL-7-induced expansion of CD8cr-CD8B-positive TcR y/6 cells which are shown to be present in the starting fetal thymocyte cell population.