Presence of calbindin D28K and GAD67 mRNAs in both orthotopic and ectopic purkinje cells of Staggerer mice suggests that Staggerer acts after the onset of cytodifferentiation
✍ Scribed by G.D. Frantz; C.W. Wuenschell; A. Messer; A.J. Tobin
- Book ID
- 102652184
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 960 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
We used in situ hybridization to study the expression of GAD,, and calbindin DZSK mRNAs in developing mouse cerebellar Purkinje cells. Both genes are expressed prenatally; calbindin D28K mRNAs can be detected in Purkinje cells of embryonic day (E) 15 mice, whereas GAD6, mRNAs first appear slightly later, in El6 mice. The stunted Purkinje cells of stuggerer (sglsg) mutant mice maintain calbindin D,,K and GAD,, expression. Our data suggest that the sghg mutation does not interfere with the transcriptional activation of these two genes, and might therefore act after the induction of specific gene expression in developing Purkinje cells.