Presence of Caffeic Acid in Flaxseed Lignan Macromolecule

✍ Scribed by Agnieszka Kosińska; Kamila Penkacik; Wiesław Wiczkowski; Ryszard Amarowicz

• Book ID: 106508396
• Publisher: Springer US
• Year: 2011
• Tongue: English
• Weight: 199 KB
• Volume: 66
• Category: Article
• ISSN: 1573-9104
• DOI: 10.1007/s11130-011-0245-1

✦ Synopsis

Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) was isolated using a semi-preparative HPLC and its structure was confirmed by LC-ESI-MS. In LM of the investigated flaxseed, one molecule of caffeic acid corresponded with five molecules of p-coumaric acid and two molecules of ferulic acid. The presence of caffeic acid in the lignan molecule might be very beneficial due to its high antioxidant activity.