Presence of an abnormalβ2-microglobulin mRNA in Daudi cells: Induction by interferon

✍ Scribed by Frédéric Rosa; Marc Fellous; Michel Dron; Michael Tovey; Michel Revel

• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 540 KB
• Volume: 17
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00364752

✦ Synopsis

Human alpha and beta interferons increase the amount of class I human histocompatibility messenger RNA HLA-A, B, C and beta 2-microglobulin in most human cells studied to date. This report concerns the effect of interferons on the Burkitt lymphoma-derived cell line Daudi, which does not express HLA-A, B, C antigens or beta 2-microglobulin on its membrane. HLA-A, B, C messenger RNA present in Daudi cells is increased by both alpha and beta interferons. Furthermore, we have shown that although it was not possible to detect mature beta 2-microglobulin protein in the cytoplasm or on the cell membrane of Daudi cells, a poly A+ messenger RNA is present in Daudi cells, which hybridizes with a cDNA clone specific for human beta 2-microglobulin. This abnormal messenger RNA is, however, increased normally by interferon. These effects were also observed with human interferon beta on a variant of Daudi cells characterized by a markedly reduced sensitivity to anti-proliferative and anti-cellular effects of human interferon alpha.