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Presence of a transposon-like element in the promoter region of an inactive patatin gene inSolanum tuberosumL.

✍ Scribed by Meike Köster-Töpfer; Wolf B. Frommer; Mario Rocha-Sosa; Lothar Willmitzer

Publisher
Springer
Year
1990
Tongue
English
Weight
673 KB
Volume
14
Category
Article
ISSN
0167-4412

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✦ Synopsis

The promoter of the PGT3 patatin gene belonging to the class II subfamily is highly homologous to other class II patatin genes except for a 736 bp insertion in front of the putative transcription start site. The insertion is characterized by structural features resembling a transposable element such as an 11 bp inverted repeat at the termini and an 8 bp duplication flanking the insertion site. Despite the high homology to active patatin genes, fusion of its promoter to the fl-glucuronidase reporter gene does not lead to detectable fl-glucuronidase (GUS) activity in transgenic potato or tobacco plants, suggesting that the inactivation of this gene might be caused by the insertion of the transposon like element.

📜 SIMILAR VOLUMES

Presence of Vi-transposon-like elements
Presence of Vi-transposon-like elements in the proopiomelanocortin gene A of Xenopus laevis does not affect gene activity
✍ Deen, Peter M.T. ;Roubos, Eric W. ;Martens, Gerard J.M. 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 372 KB

Restriction mapping of the two proopiomelanocortin (POMC) genes of the South African clawed toad Xenopus laevis revealed that POMC gene A is much larger than POMC gene B. Here we report that this size difference is mainly due to the presence of four vitellogenin (Vi)-transposon-like elements in POMC