Preparative separation of major xanthones from mangosteen pericarp using high-performance centrifugal partition chromatography

✍ Scribed by Yichu Shan; Wei Zhang

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 144 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900774

✦ Synopsis

Abstract

Mangosteen fruit pericarp (MFP) is a rich source of xanthones, which has shown remarkable pharmacological activities. To isolate xanthones, previous methods included labor intensive and time‐consuming solid‐phase extractions (Sephadex LH20, silica gel) and sequential solvent extraction. In this study, major xanthones (α‐ and γ‐mangostins) in MFP were isolated at high purity in one step utilizing high‐performance centrifugal partition chromatography with solvent system composed of petroleum ether, ethyl acetate, methanol and water (10:5:5:1). In one run, 200 mg crude extract of MFP was injected and 55.4 mg α‐mangostin and 12.4 mg γ‐mangostin were obtained with the purity of 93.6 and 98.4%, respectively. The yields of them were 86.3 and 76.3%, respectively. As α‐ and γ‐mangostins are reported to show potent antioxidant, anti‐inflammatory and anticancer activities, this method can be used for the large‐scale production of them for future in vitro and in vivo biological studies.