𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparative separation of a multicomponent peptide mixture by mass spectrometry

✍ Scribed by Xinli Yang; Philip S. Mayer; František Tureček

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
213 KB
Volume
41
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We report on the first multiplex preparative separation by mass spectrometry of bio‐organic molecules in the 200–350 Da mass range that is typical for synthetic drugs. A five‐component mixture consisting of two di‐ and three tripeptides has been separated by mass using a specially designed mass spectrometer. The instrument for preparative separations consists of an electrospray ionization (ESI) source, ion transfer optics, an electrostatic sector, and an inhomogeneous‐field magnetic mass analyzer that achieves linear mass dispersion of ion beams. Protonated peptides produced by electrospray were separated, nondestructively landed on a 16‐channel array of dry collector plates, and reconstituted in solution. The preparation procedures and the instrumental conditions have been optimized to maximize the ion currents. The significant features of the special mass spectrometer are high ion currents and simultaneous separation and collection of mixture components. Copyright © 2006 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Analysis of a Standard Peptide Mixture b
Analysis of a Standard Peptide Mixture by Capillary Electrophoresis with Mass Spectrometry and with Tandem Mass Spectrometry
✍ H. J. Major; A. E. Ashcroft 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 409 KB 👁 2 views

The combination of capillary electrophoresis with mass spectrometry allows efficient separation and identification of components in mixtures with greater specificity than can be obtained using capillary electrophoresis and ultra violet detection alone. For the mixture of peptides analysed in this ap

Separation and identification of peptide
Separation and identification of peptide mixtures in a synthesis crude of carbetocin by liquid chromatography/electrospray ionization mass spectrometry
✍ V. Sanz-Nebot; I. Toro; A. Garcés; J. Barbosa 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 2 views

In order to separate and characterize the target peptide and the side-product peptide compounds of a synthesis crude of the peptide hormone carbetocin, liquid chromatography coupled to high-flow electrospray ionization mass spectrometry (LC/ES-MS) has been used. Carbetocin is an important drug with

Sequence determination of peptides by ma
Sequence determination of peptides by mass spectrometry: Methods for combining “wet” separation techniques with mass spectrometry
✍ H. Falter; K. Jayasimhulu; R.A. Day 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 689 KB

Methods are described that allow the combination of established techniques for peptide separation, paper chromatography and electrophoresis, with mass spectrometry. The development of these methods is part of an ongoing effort in the search for a methodology for the systematic utilization of mass sp