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Preparative-scale, recirculating, pH-biased binary isoelectric trapping separations

โœ Scribed by Evan Shave; Gyula Vigh

Book ID
102183771
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
101 KB
Volume
25
Category
Article
ISSN
0173-0835

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Two approaches are described in this paper for the synthesis of isoelectric buffers that have pI values in the 1.5 < pI < 4.3 range. The first synthesis relies on the alkylation of existing aminodicarboxylic acids and recovery of the ampholyte as an inner salt. The second synthesis method forms low-