Following previous studies of the use of non-porous monodisperse 1.5-pm noctyl-and n-octadecyl-bonded silicas in gradient elution of proteins, this work was aimed at elucidating further the properties of this novel column material for peptide and protein separations in comparison with wide-pore sili
โฆ LIBER โฆ
Preparative reversed-phase liquid chromatography of peptides: Isocratic two-step elution system for high loads on analytical columns
โ Scribed by Anthony R Mehok; Colin T Mant; Lajos Gera; John Stewart; Robert S Hodges
- Book ID
- 108339078
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 911 KB
- Volume
- 972
- Category
- Article
- ISSN
- 1873-3778
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A procedure, developed for the cleavage and reversible blocking of disulfide bonds of proteins by S-sulfonation in preparation for peptide mapping, was applied to ribonuclease A. The complete peptide maps of sulforibonuclease A using limited Staphylococcus aureus protease digestion, tryptic digestio