High-speed preparative reversed-phase hi
โ
Keith Ashman; Armin Bosserhoff; Rainer Frank
๐
Article
๐
1987
๐
Elsevier Science
๐
English
โ 261 KB
A rapid method is described for the purification and analysis of synthetic oligonucleotides, based on reversed-phase high-performance liquid chromatography. Volatile buffers and a short column (40 mm X 4.6 mm) packed with Nucleosil 300-5 C4 were employed. Monitoring the column effluent with an UV de