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Preparative Purification of a Recombinant Protein by Hydrophobic Interaction Chromatography: Modulation of Selectivity by the Use of Chaotropic Additives

✍ Scribed by Abhinav A. Shukla; Joshua Peterson; Laura Sorge; Patsy Lewis; Shannon Thomas; Steve Waugh

Book ID
109386464
Publisher
American Institute of Chemical Engineers
Year
2002
Tongue
English
Weight
192 KB
Volume
18
Category
Article
ISSN
8756-7938

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On the use of mild hydrophobic interacti
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## Abstract The behavior of a series of pure proteins partitioned in aqueous two‐phase systems is compared with their behavior during mild hydrophobic interaction chromatography (HIC). A simple theoretical rationale for this comparison is presented based upon solvophobic theory. Similarities were f