✦ LIBER ✦
Preparative Asymmetric Reduction of 3-Ketobutyrate and -valerate by Suspended Cells of Thermophilic Bacteria (Thermoanaerobium brockii) in Ordinary Laboratory Equipment
✍ Scribed by Dieter Seebach; Fabio Giovannini; Bernd Lamatsch
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- German
- Weight
- 212 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0018-019X
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✦ Synopsis
Abstract
The thermophilic and anaerobic bacteria specified in the title are isolated on a 0.8 kg scale by tangential flow filtration and centrifugation from a 300‐1 bioreactor. The microorganisms are stored in a freezer (−20°) and used, analogously to baker's yeast, for asymmetric reductions. Thus, ethyl 3‐ketovalerate (4.3) g/1 (H~2~O) is converted in 40% yield to (S)‐3‐hydroxyvalerate (6), with an enantiomeric excess of 93% (24 h at 72°).