Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide
✍ Scribed by G. R. Seely; M. J. Duncan; W. E. Vidaver
- Publisher
- Springer-Verlag
- Year
- 1972
- Tongue
- English
- Weight
- 764 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0025-3162
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✦ Synopsis
Dimethyl sulfoxide (D1KSO) rapidly extracts much of the chlorophyll v and fucoxanthin from the intact thalli of brown algae. Subsequent extraction by acetone rapidly removes most of the chlorophyll a and fl-carotene. An analytical procedure for these pigments has been developed, based upon this extraction sequence, followed by partition of the acetone extract between hexane and aqueous acetone. Fueoxanthin and chlorophyll c can be recovered from the DMSO extract and the aqueous acetone phase by extraction into ethyl acetate and subsequent evaporation. The DMSO-acetone extraction sequence is also effective with green algae and a few red algae, although the full scope of the method has not been investigated.