Preparation Techniques of Small Sample Sizes for Sulphur and Indirect Total Glucosinolate Analysis inBrassica Seeds by X-Ray Fluorescence Spectroscopy
✍ Scribed by Schnug, E. ;Murray, Fiona ;Haneklaus, Silvia
- Book ID
- 102933377
- Publisher
- John Wiley and Sons
- Year
- 1993
- Weight
- 689 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0931-5985
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✦ Synopsis
Abstract
Two new preparation techniques for Brassica seeds are introduced, which enable the determination of total sulphur concentrations and indirectly the total glucosinolate concentrations in small sample sizes and single seeds by means of X‐ray fluorescence spectroscopy. Small sample sizes, down to 250 mg, are milled in peppermill type grinders and prepared as thin layers in liquid cuvettes. Single seeds are embedded by means of a sulphur free glue based on acetylated glucose in wax tablets and cut into halves. Measurement and calibration for small sample sizes are in accordance with the rules for the original X‐RF method. In case of single seeds the area of the cut surface need to be estimated and the collected S‐Kα intensities corrected for the active fluorescent area of the standard reference materials.