Preparation, quality control and stability of 99mTc-sparafloxacin complex, a novel agent for detecting sites of infection

✍ Scribed by M. A. Motaleb

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: French
• Weight: 119 KB
• Volume: 52
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.1619

✦ Synopsis

Abstract

Labeling of sparafloxacin with technetium‐99m using stannous chloride as a reducing agent was investigated. Dependence of the yield of ^99m^Tc‐sparafloxacin complex on the concentration of sparafloxacin, reducing agent, pH and reaction time was studied. Under optimum conditions, the labeling yield of ^99m^Tc‐sparafloxacin complex (95%) was achieved by using 2.5 mg of sparafloxacin, 50 µg of Sn(II), pH 10 and 30‐min reaction time. ^99m^Tc‐sparafloxacin complex was stable for 3 h after labeling, then the yield decreased gradually to 81.9% at 6 h. Biodistribution studies in rats were carried out in experimentally induced infection in the left thigh using Staphylococcus aureus. The ratios of bacterial infected thigh/contralateral thigh were then evaluated. The time for the maximum accumulation of ^99m^Tc‐sparafloxacin at the site of the infection was 30 min after the administration followed by gradual decline. The abscess‐to‐muscle ratio for ^99m^Tc‐sparafloxacin was 5.9±0.7, while that for the commercially available ^99m^Tc‐ciprofloxacin was 3.8±0.5 under the same experimental paradigm, indicating that ^99m^Tc‐sparafloxacin could be used for infection imaging. Copyright © 2009 John Wiley & Sons, Ltd.