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Preparation of15N-Labeled Phytoplankton Samples for Optical Emission Spectrometry

✍ Scribed by S. Kristiansen and E. Paasche


Book ID
125130984
Publisher
Association for the Sciences of Limnology and Oceanography
Year
1982
Tongue
English
Weight
409 KB
Volume
27
Category
Article
ISSN
0024-3590

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The preparation of 13C-15N-labeled nucle
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Chlorella pyrenoidosa can be grown in 90.8 atom% \*"COP and 99. I atom% K15N0, to yield algae with substitution of 82 to 86% 'VI for 'YJ and 92 to 96% 15N for \*\*N. Methods are described for extracting preparative amounts of ribonucleosides which can be used directly for density labeling RNA in euk