Preparation of X-ray microanalytical samples by means of freeze-drying, fixation and embedding under vacuum

Use of freeze-substitution and molecular

Use of freeze-substitution and molecular distillation drying in the preparation of Dunaliella parva for ion localization studies by X-ray microanalysis

✍ Dr. M. A. Hajibagheri; T. J. Flowers 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 593 KB

The determination of ion concentrations within cells and sub-cellular compartments remains a difficult procedure, as the volumes to be analyzed are rather small. X-ray microanalysis is sufficiently sensitive, and has adequate resolution, to measure these concentrations. The major difficulties are re