Preparation of weak cation exchange packings based on monodisperse poly(chloromethylstyrene-co-divinylbenzene) particles and its chromatographic properties

✍ Scribed by Yin-Mao Wei; Qiang Chen; Xin-Du Geng

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 532 KB
• Volume: 19
• Category: Article
• ISSN: 0256-7660
• DOI: 10.1002/cjoc.20010191117

✦ Synopsis

Abstract

Monodisperse poly (chloromethylstyrene‐co‐divinylbenzene) particles were firstly prepared by a two‐step swelling method. Based on this media, one kind of weak cation ion exchange packings was prepared. It was demonstrated that the prepared packings have comparative advantages for biopolymer separation with high column efficiency, low interstitial volume and low column backpressure, and have good resolution to proteins. The effects of salt concentration and pH of mobile phase on protein retentions were investigated. The properties of the weak cation ion exchange packings were evaluated by the unified retention model for mixed‐mode interaction mechanism in ion exchange and hydrophobic interaction chromatography.