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Preparation of Vesicular Stomatitis Virus-G (VSV-G) Conjugate and Its Use in Gene Transfer

✍ Scribed by Miyanohara, A.


Book ID
118237651
Publisher
Cold Spring Harbor Laboratory Press
Year
2012
Tongue
English
Weight
214 KB
Volume
2012
Category
Article
ISSN
1940-3402

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Preparation of Vesicular Stomatitis Viru
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The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells tran

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## Abstract ## Background The involvement of surface molecules in HIV‐1‐derived lentivirus (LV)‐mediated transduction of airway epithelial cells has not been studied so far. The present study aimed to evaluate the role of glycosaminoglycans (GAGs) in gene transfer mediated by a third generation ve