The purification of trypsin from bovine pancreas was employed in a case study concerning the design and optimization of peptide-ligand adsorbents for affinity chromatography. Four purpose-designed tripeptide-ligands were chemically synthesized (>95% pure), exhibiting an Arg residue as their C-termin
โฆ LIBER โฆ
Preparation of trypsin-immobilised chitosan beads and their application to the purification of soybean trypsin inhibitor
โ Scribed by Li Zhang; Bin Zhang; Hong Lin; Pei-Pei Liu; Li-Na Yu; Dong-Feng Wang
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 293 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0022-5142
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New effective potentials acting between pairs of residues in proteins are proposed based on statistics of average distances and standard deviations between C atoms of residues in protein tertiary structures. Gaussian functions are adopted as analytical forms of the potentials. A protein structure is