## Abstract An overview is given of the possibilites of ^3^H NMR in the characterisation of ^3^Hโlabelled compounds. This technique gives information on the identity of the tritiated compounds, the position of the tritium, the distribution of the label and even the radiochemical purity of the label
Preparation of tritiated monomers and analysis of the tritium distribution by 3H-NMR spectroscopy
โ Scribed by R.J. Diamond; J.R. Jones; W.W. Wright
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 334 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-3057
No coin nor oath required. For personal study only.
โฆ Synopsis
A number of monomers have been tritiated to reasonably high specific activity (in the range 2-30 mCi/mmol) using a variety of synthetic and catalytic procedures and the tritium distribution in the products analyzed by 3H-NMR spectroscopy.
๐ SIMILAR VOLUMES
Synthetic melatonin was iodinated by treatment with potassium iodide in the presence of an oxidizing agent, Iodo-Gen. The iodination products of melatonin were extracted with chloroform and separated by HPLC. The fraction showing immunoreactivity with respect to melatonin antisera was characterized
## Abstract The preferred solute conformation of ethyl 3ฮฑโphenyltropaneโ3ฮฒโcarboxylate hydrochloride, the tropane analogue of pethidine, is shown to be a piperidine chair with an axial 3โphenyl substituent by analysis of its ^1^H NMR characteristics and spectral comparisons with model compounds. Co
A simple method to tritiate commercial samples of heparin is described. Heparin is reacted with NaB3H4 at pH 8.0 for 3 h at room temperature, after which the 3H-labeled mucopolysaccharide is isolated by gel filtration. Using NaB3& of low specific radioactivity (227-555 mCi/mmol), [3H]heparins contai
## Abstract Pushโpull alkenes with variable structure were investigated with respect to the influence of the different substituents at the central C,C double bond on the dynamic behaviour of this type of compound. ^1^H and ^13^C NMR spectroscopic investigations were carried out for keteneโ__S__,__S
COSY and NOESY spectra (400 MHz) have been used to complete the assignment of the 'H NMR spectra of 1and 3-nitrobenzo[e]pyrene. Both compounds show strong bay region nuclear Overhauser enhancement and significant coupling across bay regions. Both compounds also exhibit extensive long-range couplings