Preparation of the iodine-124 derivative of the Bolton–Hunter reagent ([124I]I-SHPP) and its use for labelling a VEGF antibody as a PET tracer
✍ Scribed by Matthias Glaser; Veronica A. Carroll; David R. Collingridge; Eric O. Aboagye; Pat Price; Roy Bicknell; Adrian L. Harris; Sajinder K. Luthra; Frank Brady
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- French
- Weight
- 128 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0022-2135
- DOI
- 10.1002/jlcr.634
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✦ Synopsis
Abstract
This study describes the radioiodination of an antibody specific to the vascular endothelial growth factor (VEGF), VG76e, with [^124^I]iodine to obtain a novel PET tracer for measurement of angiogenesis. In vitro binding assays showed a significantly higher immunoreactive fraction with the protein labelling reagent N‐succinimidyl 3‐(4‐hydroxy‐5‐[^125^I]iodophenyl) propionate ([^125^I]Bolton–Hunter reagent, [^125^I]I‐SHPP) (34.0±4.0%) as compared with __N‐__succinimidyl 3‐[^125^I]iodobenzoate (10.9±6.4%) or direct radioiodination using [^125^I]iodide and IodoGen (3.1±3.0%). Consequently, the cyclotron–produced positron–emitting [^124^I]iodine (T~1/2~=4.2 days) was employed to prepare [^124^I]I‐SHPP. Using an improved radioiodination methodology, [^124^I]I‐SHPP was prepared from sodium [^124^I]iodide with IodoGen at pH 6.5. The [^124^I]Bolton–Hunter reagent was isolated with 25–58% (n=3) radiochemical yield and 88–95% (n=3) radiochemical purity by the conventional extraction procedure. The conjugate of VG76e with [^124^I]I‐SHPP was prepared with 17–18% (__n=__3) labelling efficiency and 98% radiochemical purity. The immunoreactive fraction was determined to be 33.5% (n=2). Copyright © 2002 John Wiley & Sons, Ltd.