Preparation of technetium-99 m human albumin nanospheres for use in the measurement of reticuloendothelial clearance capacity

✍ Scribed by Alistair M. Millar; Martin Bodingbauer; Lesley M. O'Brien; Lynn A. Beattie; Rodger Duffin; Stephen J. Wigmore

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: French
• Weight: 97 KB
• Volume: 54
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.1861

✦ Synopsis

Background:

The rate at which radioactive albumin particles clear from the bloodstream is used to measure reticuloendothelial capacity. Traditionally, iodine radioisotopes have been used. Non-active albumin particles mixed with the technetium-99 m labelled particles of the readily available radiopharmaceutical 99m Tc Albumin Nanocolloid (ANC) would be more convenient. Aim: To prepare human albumin nanospheres (HAN) with a diameter similar to 99m Tc-ANC and to investigate their stability. Experimental: HAN were prepared by heating 30 g/l albumin solution pH 10 at 701C for 20 min then 751C for 17.5 min, adjusting to pH 7.5 and sterilizing by filtration. Seven batches were prepared. The HAN diameter was measured by photon correlation spectroscopy at 0-3 months. Various measures of stability were performed. Results: The mean diameter of the HAN was 3.971.9, 4.071.5, 4.471.6 and 4.272.0 nm at 0, 1, 2 and 3 months. After mixing HAN and 99m Tc-ANC, no changes in particle size or radiolabelling of ANC were detected. Conclusions: HAN with a diameter comparable to the particles in 99m Tc-ANC were prepared successfully. The diameter of HAN does not change over 3 months or after mixing with 99m Tc-ANC. The particle size and labelling efficiency of 99m Tc-ANC are not affected by the addition of HAN.