Preparation of silicone-coated 5–25-μm I.D. fused-silica capillary columns for open-tabular liquid chromatography

✍ Scribed by O. van Berkel-Geldof; J.C. Kraak; H. Poppe

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 825 KB
• Volume: 499
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96984-x

✦ Synopsis

Fused-silica capillaries of I.D. of 5, 10 and 25 pm were coated with PS-255 or PS-264 in order to achieve highly efficient columns for reversed-phase open-tubular liquid chromatography.

Various coating solvents were tested, of which Freon-l 14 showed the most promising features with respect to speed of coating and ease of handling. The highest efficiency was obtained with a 1 m x 5.0 pm I.D. column, showing 250 000 and 58 800 plates for solutes with capacity factors 0.2 and 2.6, respectively, at approximately six times the optimum linear velocity.