Abstract
Two molecularly imprinted polymers (MIPs) were prepared using (S)โibuprofen as the template molecule as well as methacrylic acid (MAA) or 4โvinylpyridine and ethylene glycol dimethacrylate (EGDMA) as the functional monomer and crosslinker, respectively. Free radical polymerization was carried out at 4ยฐC under ultraviolet (UV) radiation. The MIPs thus obtained were ground into 25โ44 ฮผm, which were slurry packed into analytical columns. The template molecules were removed by acetic acid/methanol solution (1:9, v/v). highโperformance liquid chromatography (HPLC), with UV detection, was used to evaluate the binding performance of the MIP for the template. The selectivity of (S)โibuprofen and naproxen on the hostโguest system were assessed using acetonitrileโbased mobile phases. The limits of detection of ibuprofen and naproxen were found to be 0.1844 mmol/L and 0.3264 mmol/L, while the limits of quantitation were 0.6262 mmol/L and 1.0909 mmol/L, respectively. The stationary phase was applied successfully to the commercial tablet analysis. Ibuprofen and naproxen were extracted from tablets with acetonitrile; analysis results showed a good relative standard deviation (RSD) of 0.81โ1.24% and accuracy from โ4.01 to +2.98% for ibuprofen as well as an RSD of 0.59โ0.86% and accuracy from โ4.01 to โ2.01% for naproxen. ยฉ 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 2972โ2979, 2006