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Preparation of Recombinant -Thrombin: High-Level Expression of Recombinant Human Prethrombin-2 and Its Activation by Recombinant Ecarin

✍ Scribed by Yonemura, Hiroshi (author);Imamura, Takayuki (author);Soejima, Kenji (author);Nakahara, Yo (author);Morikawa, Wataru (author);Ushio, Yoshitaka (author);Kamachi, Yasuharu (author);Nakatake, Hiroshi (author);Sugawara, Keishin (author);Nakagaki, Tomohiro (author);Nozaki, Chikateru (author)

Book ID
121342416
Publisher
Oxford University Press
Year
2004
Tongue
English
Weight
223 KB
Volume
135
Category
Article
ISSN
0021-924X

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## Abstract We have expressed active human cathepsin S to 60 mg/L in Sf9 cells using a baculovirus system. Production of milligram quantities has facilitated crystallographic studies to determine the structure of this enzyme, which has unique properties among lysosomal cysteine proteinases. Recombi