Preparation of Oriented Lipid Bilayer on Ultrathin Polymers for Solid-State NMR Analyses of Peptide–Membrane Interactions

Three-Dimensional Solid-State NMR Spectr

Three-Dimensional Solid-State NMR Spectroscopy Is Essential for Resolution of Resonances from In-Plane Residues in Uniformly 15N-Labeled Helical Membrane Proteins in Oriented Lipid Bilayers

✍ Francesca M Marassi; Che Ma; Jennifer J Gesell; Stanley J Opella 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 188 KB

Uniformly 15 N-labeled samples of membrane proteins with helices aligned parallel to the membrane surface give two-dimensional PISEMA spectra that are highly overlapped due to limited dispersions of 1 H-15 N dipolar coupling and 15 N chemical shift frequencies. However, resolution is greatly improve