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Preparation of organotypic hippocampal slice cultures: interface method

✍ Scribed by De Simoni, Anna; MY Yu, Lily


Book ID
109945855
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
280 KB
Volume
1
Category
Article
ISSN
1750-2799

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We have examined the functional characteristics of microglia in an environment where the cytoarchitecture of the brain is preserved. Using organotypic slice culture under serum-free conditions, microglia initially demonstrated a rounded morphology but after 10 days in vitro (DIV), microglia in the s