Preparation of lipid vesicles containing high levels of entrapped radioactive cations

✍ Scribed by Marcia R. Mauk; Ronald C. Gamble

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 486 KB
• Volume: 94
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90364-6

✦ Synopsis

The ionophore A23 187 was incorporated into the lipid bilayer of unilamellar vesicles and used to carry externally added indium ions to a chelator, nitrilotriacetic acid, which was previously entrapped in the vesicles. The extent of vesicle loading was assayed by Sephadex chromatography and y-ray perturbed angular correlation spectroscopy. Incubation conditions were developed which allow rapid loading of more than 90% of added lllIns+ with specific activities of ZOO-300 &i/mg lipid.