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Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments

โœ Scribed by Ylva M. Selin; Barbara Harich; J. L. Johnson

Publisher
Springer
Year
1983
Tongue
English
Weight
733 KB
Volume
8
Category
Article
ISSN
0343-8651

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โœ V.V. Didenko ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 389 KB

We have used M13 single-stranded DNA bound by uv to small pieces of nylon membrane for the synthesis of biotinylated single-stranded DNA probes. The labeling method requires a large fragment of DNA polymerase I and random hexanucleotides. There is no need for previous linearization of the template.