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Preparation of intercalating dye thiazole orange and derivatives

โœ Scribed by Todor G. Deligeorgiev; Nikolai I. Gadjev; Karl-Heinz Drexhage; Ram W. Sabnis

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
325 KB
Volume
29
Category
Article
ISSN
0143-7208

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The interaction of the fluorescent dye thiazole orange (TO) with nucleic acids is characterized. It is found that TO binds with highest affinity to double-stranded (ds) DNA [log(K) ร‰ 5.5 at 100 mM salt], about 5-10 times weaker to single-stranded polypurines, and further 10-1000 times weaker to sing