✦ LIBER ✦

Preparation of human immune effector T cells containing iron-oxide nanoparticles

✍ Scribed by Hironori Iida; Kosuke Takayanagi; Takuya Nakanishi; Akiko Kume; Kouji Muramatsu; Yoshio Kiyohara; Yasuto Akiyama; Tetsuya Osaka

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 365 KB
Volume: 101
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.21992

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Preparation of human immune T cells containing iron‐oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs. Furthermore, the effects of dosages and diameter of magnetite nanoparticles on the magnetite incorporation were investigated, and it was demonstrated that the increase in dosage promoted the incorporation of nanoparticles and the uptake into PBLs was more effective for magnetite nanoparticles, which formed smaller aggregations in medium. Finally, the demonstration of magnetite incorporation into enriched T cells and tumor antigen‐specific cytotoxic T lymphocyte (CTL) line promises the achievement of magnetically mediated immunotherapy with tumor‐specific CTLs containing magnetic nanoparticles. Biotechnol. Bioeng. © 2008 Wiley Periodicals, Inc.

📜 SIMILAR VOLUMES

Preparation of Superparamagnetic Dextran

Preparation of Superparamagnetic Dextran-coated Iron Oxide Nanoparticles used as a Novel Gene Carrier into Human Bladder Cancer Cells

✍ Zhengguo CAO; Siwei ZHOU; Jihong LIU; Xiaodong SONG 📂 Article 📅 2005 🏛 Springer 🌐 English ⚖ 195 KB

The promotion of human mesenchymal stem

The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles

✍ Dong-Ming Huang; Jong-Kai Hsiao; Ying-Chun Chen; Li-Ying Chien; Ming Yao; Yin-Ka 📂 Article 📅 2009 🏛 Elsevier Science 🌐 English ⚖ 439 KB

Superparamagnetic iron oxide (SPIO) nanoparticles are very useful in cell imaging; meanwhile, however, biosafety concerns associated with their use, especially on therapeutic stem cells, have arisen. Most studies of biosafety issues focus on whether the nanoparticles have deleterious effects. Here,

In vitro labeling of human umbilical cor

In vitro labeling of human umbilical cord mesenchymal stem cells with superparamagnetic iron oxide nanoparticles

✍ Sheng-Li Hu; Jiu-Quan Zhang; Xiang Hu; Rong Hu; Hai-Shui Luo; Fei Li; Yong-Zhi X 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 302 KB 👁 2 views

## Abstract Human umbilical cord mesenchymal stem cells (hUC‐MSCs) transplantation has been shown to promote regeneration and neuroprotection in central nervous system (CNS) injuries and neurodegenerative diseases. To develop this approach into a clinical setting it is important to be able to follo

Human afferent lymph from normal skin co

Human afferent lymph from normal skin contains an increased number of mainly memory / effector CD4+ T cells expressing activation, adhesion and co-stimulatory molecules

✍ Nikhil Yawalkar; Robert E. Hunger; Werner J. Pichler; Lasse R. Braathen; Christo 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 204 KB 👁 2 views

We investigated the T cell population in the afferent lymph and the peripheral blood with regard to expression of activation, adhesion and co-stimulatory molecules and cytokine profile by immunohistochemistry and flow cytometry. The majority of the lymphoid cell population in the afferent lymph were