𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation of highly magnetic chitosan particles and their use for affinity purification of enzymes

✍ Scribed by Dr Xiaoning An; Zhixing Su; Hanmin Zeng

Publisher
Wiley (John Wiley & Sons)
Year
2003
Tongue
English
Weight
94 KB
Volume
78
Category
Article
ISSN
0268-2575

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A novel protocol for preparation of highly magnetic chitosan particles, with a coercive force as high as 3500 Oe, has been developed. The surface of the particles was functionalized with aldehyde groups to facilitate the attachment of affinity ligands. The optimum conditions for the preparation of highly magnetic chitosan particles and immobilization of trypsin on magnetic particles were obtained; these particles were then used for affinity purification of aprotinin, and the conditions of affinity purification are discussed in detail. The proposed method was successfully applied to the affinity purification of aprotinin. Copyright Β© 2003 Society of Chemical Industry

πŸ“œ SIMILAR VOLUMES

The synthesis of sub-micron magnetic par
The synthesis of sub-micron magnetic particles and their use for preparative purification of proteins
✍ Hwee Peng Khng; David Cunliffe; Stephen Davies; Nigel A. Turner; Evgeny N. Vulfs πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 170 KB πŸ‘ 2 views

A novel one-step protocol for the preparation of sub-micron magnetic particles as small as 30 nm in diameter has been developed. The surface of the particles was functionalized with carboxyl groups (∼1 COOH per 15Γ… 2 ) to facilitate the attachment of affinity ligands. The high surface area of the re

Use of the unique properties of fluoropo
Use of the unique properties of fluoropolymers in the design of new, highly effective, ecologically friendly technologies for the fine purification of hydrogen halides and their aqueous solutions
✍ G. Z. Blum; E. A. Ryabenko; A. M. Yaroshenko; G. E. Zaikov; V. G. Zaikov πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 117 KB πŸ‘ 2 views

## Abstract A number of properties of fluoropolymers in aqueous solutions of hydrogen halides were investigated. The results enable fluoropolymers to be used in the purification of hydrogen halides and their aqueous solutions. The hydrophobicity of fluoropolymers and their diffusion permeability en