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Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

✍ Scribed by Woods, Georgia (author);Zito, Karen (author)

Book ID
118047880
Publisher
Journal of Visualized Experiments
Year
2008
Tongue
English
Weight
99 KB
Volume
12
Category
Article
ISSN
1940-087X

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## Abstract We report here that differentiated, primary, postmitotic neurons and photoreceptors in cultures obtained from embryonic chicks can express foreign genes after transfection by the calcium phosphate method. A variety of viral promoters were tested by using either β‐galactosidase or chlora