Preparation of Fritless Packed Silica Columns for Capillary Electrochromatography

✍ Scribed by Fujimoto, Chuzo

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 59 KB
• Volume: 23
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/(sici)1521-4168(20000101)23:1<89::aid-jhrc89>3.0.co;2-5

✦ Synopsis

Fritless packed silica gel columns were prepared using sol-gel technology. A part of a 75 lm i.d. fused silica capillary was filled with a mixture of tetramethoxysilane and poly(ethylene glycol). After gelling at 40 8C and heating at 300 8C, the resultant silica gel was derivatized with dimethyloctadecylchlorosilane. A scanning electron micrograph of a cross-section of the capillary column showed that the gel took the form of a spherical particle aggregate and adhered to the column inner wall. The column performance was evaluated for electrochromatography using acetonitrile -50 mM HEPES buffer (pH 6.6) (60/40 or 40/60, v/v) as the mobile phase. An electroosmotic flow of 1.0 mm/s was generated with (60/40, v/v) acetonitrile/HEPES buffer at a field strength of 546 V/cm. Using a sol-gel-derived packed column at an electroosmotic flow of 0.5 mm/s, efficiencies of up to 1.1610 5 plates/m were obtained for retained solutes.