Preparation of crystalline bovine liver ?-glucuronidase

✍ Scribed by Bonnichsen, Roger ;Heikkinen, Irma

• Book ID: 104662479
• Publisher: Springer-Verlag
• Year: 1978
• Tongue: English
• Weight: 461 KB
• Volume: 81
• Category: Article
• ISSN: 1437-1596
• DOI: 10.1007/bf00203868

✦ Synopsis

A method is described for the preparation of pure /3-glucuronidase from bovine liver. The procedure includes ammonium sulfate, acetone and ethanol fractionation and a simple two-step ion exchange chromatography. The yield is acceptable and the method requires only standard laboratory equipment. The pure enzyme is easily crystallized from ammonium sulfate. Some practical applications of the pure ~3-glucuronidase are discussed.