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Preparation of collagen substrates for cell attachment: Effect of collagen concentration and phosphate buffer

✍ Scribed by Hynda K. Kleinman; Ermona B. McGoodwin; Stephen I. Rennard; George R. Martin

Book ID
102984349
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
995 KB
Volume
94
Category
Article
ISSN
0003-2697

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✦ Synopsis

We have studied the attachment of cultured Chinese hamster ovary cells to collagen substrates prepared in several ways. The attachment of these cells to collagen required under most conditions either serum or tibronectin purified from serum. Reconstituted collagen substrates required greater amounts of tibronectin than dishes coated by drying a collagen solution, but in each case the amount of fibronectin required was proportional to the amount of collagen on the dish. High levels of phosphate, 0.01 M and above, used as a buffer in heat-reconstituted collagen substrates allowed cell attachment without fibronectin. However, since the cells did not spread under these conditions and were not released from the substrate when incubated with trypsin, binding of cells with such levels of phosphate probably represents nonphysiological adhesion.

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