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Preparation of Chromatin : Variation in the Template Properties of Chromatin Dependent on the Method of Preparation

✍ Scribed by David I. de POMERAI; Peter H. W. BUTTERWORTH; C. James CHESTERTON


Book ID
115114254
Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
978 KB
Volume
46
Category
Article
ISSN
1432-1327

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Hydrodynamic shearing of chromatin in the presence of Mg(2+) ions produces two discrete types of particles: (1) molecules of completely free DNA which comprise 20-23% of the total DNA and (2) histone-covered DNA molecules which contain all five histone fractions. The average length of free DNA molec